Original articles:
- Shine-Dalgarno Accessibility Governs Ribosome Binding to the Adenine Riboswitch
Blechar J, de Jesus V, Fürtig B, Hengesbach M, Schwalbe H.
ACS Chem Biol. 2024;19(3):607-618.
doi:10.1021/acschembio.3c00435 - Site-Specific Labeling of RNAs with Modified and 19F-Labeled Nucleotides by Chemo-Enzymatic Synthesis.
Chemistry. 2023 May 2;29(25):e202203368.
doi: 10.1002/chem.202203368 - Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug Development.
Angew Chem Int Ed Engl. 2022 Nov 14;61(46):e202205858.
doi: 10.1002/anie.202205858 -
Combining Coarse-Grained Simulations and Single Molecule Analysis Reveals a Three-State Folding Model of the Guanidine-II Riboswitch.Front Mol Biosci. 2022 Apr 19;9:826505.
doi: 10.3389/fmolb.2022.826505 - Structural analysis of temperature-dependent alternative splicing of HsfA2 pre-mRNA from tomato plants.
RNA Biol. 2022;19(1):266-278.
doi: 10.1080/15476286.2021.2024034 - Exploring the druggability of conserved RNA regulatory elements in the SARS-CoV-2 genome.
Sreeramulu S, Richter C, Berg H, Wirtz Martin MA, Ceylan B, Matzel T, Adam J, Altincekic N, Azzaoui K, Bains JK, Blommers MJJ, Ferner J, Fürtig B, Göbel M, Grün JT, Hengesbach M, Hohmann KF, Hymon D, Knezic B, Martins J, Mertinkus KR, Niesteruk A, Peter SA, Pyper DJ, Qureshi NS, Scheffer U, Schlundt A, Schnieders R, Stirnal E, Sudakov A, Tröster A, Vögele J, Wacker A, Weigand JE, Wirmer-Bartoschek J, Wöhnert J, Schwalbe H.
Angew Chem Int Ed Engl. 2021 Jun 23.
DOI: 10.1002/anie.202103693. - Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
Altincekic N*, Korn SM*, Qureshi NS*, Dujardin M*, Ninot-Pedrosa M*, Abele R, Abi Saad MJ, Alfano C, Almeida FCL, Alshamleh I, de Amorim GC, Anderson TK, Anobom CD, Anorma C, Bains JK, Bax A, Blackledge M, Blechar J, Bockmann A#, Brigandat L, Bula A, Butikofer M, Camacho-Zarco AR, Carlomagno T, Caruso IP, Ceylan B, Chaikuad A, Chu F, Cole L, Crosby MG, de Jesus V, Dhamotharan K, Felli IC, Ferner J, Fleischmann Y, Fogeron ML, Fourkiotis NK, Fuks C, Furtig B, Gallo A, Gande SL, Gerez JA, Ghosh D, Gomes-Neto F, Gorbatyuk O, Guseva S, Hacker C, Hafner S, Hao B, Hargittay B, Henzler-Wildman K, Hoch JC, Hohmann KF, Hutchison MT, Jaudzems K, Jovic K, Kaderli J, Kalnins G, Kanepe I, Kirchdoerfer RN, Kirkpatrick J, Knapp S, Krishnathas R, Kutz F, Zur Lage S, Lambertz R, Lang A, Laurents D, Lecoq L, Linhard V, Lohr F, Malki A, Bessa LM, Martin RW, Matzel T, Maurin D, McNutt SW, Mebus-Antunes NC, Meier BH, Meiser N, Mompean M, Monaca E, Montserret R, Marino Perez L, Moser C, Muhle-Goll C, Neves-Martins TC, Ni X, Norton-Baker B, Pierattelli R, Pontoriero L, Pustovalova Y, Ohlenschlager O, Orts J, Da Poian AT, Pyper DJ, Richter C, Riek R, Rienstra CM, Robertson A, Pinheiro AS, Sabbatella R, Salvi N, Saxena K, Schulte L, Schiavina M, Schwalbe H#, Silber M, Almeida MDS, Sprague-Piercy MA, Spyroulias GA, Sreeramulu S, Tants JN, Tars K, Torres F, Tows S, Trevino MA, Trucks S, Tsika AC, Varga K, Wang Y, Weber ME, Weigand JE, Wiedemann C, Wirmer-Bartoschek J, Wirtz Martin MA, Zehnder J, Hengesbach M# and Schlundt A#
Front Mol Biosci, 2021, 8, 653148.
doi: 10.3389/fmolb.2021.653148 - Eukaryote specific RNA and protein features facilitate assembly and catalysis of H/ACA snoRNPs
Trucks S*, Hanspach G* and Hengesbach M#
Nucleic Acids Res, 2021, 49, 4629-4642.
doi: 10.1093/nar/gkab177 - The protective role of m1A during stress-induced granulation
Alriquet M, Calloni G, Martinez-Limon A, Delli Ponti R, Hanspach G, Hengesbach M, Tartaglia GG and Vabulas RM
J Mol Cell Biol, 2021, 12, 870-880.
doi: 10.1093/jmcb/mjaa023 - Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy
Wacker A*, Weigand JE*, Akabayov SR, Altincekic N, Bains JK, Banijamali E, Binas O, Castillo-Martinez J, Cetiner E, Ceylan B, Chiu LY, Davila-Calderon J, Dhamotharan K, Duchardt-Ferner E, Ferner J, Frydman L, Furtig B, Gallego J, Grun JT, Hacker C, Haddad C, Hahnke M, Hengesbach M, Hiller F, Hohmann KF, Hymon D, de Jesus V, Jonker H, Keller H, Knezic B, Landgraf T, Lohr F, Luo L, Mertinkus KR, Muhs C, Novakovic M, Oxenfarth A, Palomino-Schatzlein M, Petzold K, Peter SA, Pyper DJ, Qureshi NS, Riad M, Richter C, Saxena K, Schamber T, Scherf T, Schlagnitweit J, Schlundt A, Schnieders R, Schwalbe H, Simba-Lahuasi A, Sreeramulu S, Stirnal E, Sudakov A, Tants JN, Tolbert BS, Vogele J, Weiss L, Wirmer-Bartoschek J, Wirtz Martin MA, Wohnert J and Zetzsche H
Nucleic Acids Res, 2020, 48, 12415-12435.
doi: 10.1093/nar/gkaa1013 - Structural dynamics govern substrate recruitment and catalytic turnover in H/ACA RNP pseudouridylation
Schmidt A*, Hanspach G* and Hengesbach M#
RNA Biol, 2020, 1-10.
doi: 10.1080/15476286.2020.1842984 - RNA secondary structure dependence in METTL3-METTL14 mRNA methylation is modulated by the N-terminal domain of METTL3
Meiser N, Mench N and Hengesbach M#
Biol Chem, 2020, 402, 89-98.
doi: 10.1515/hsz-2020-0265 - Site-Specific Detection of Arginine Methylation in Highly Repetitive Protein Motifs of Low Sequence Complexity by NMR
Altincekic N*, Lohr F, Meier-Credo J, Langer JD, Hengesbach M, Richter C and Schwalbe H#
J Am Chem Soc, 2020, 142, 7647-7654.
doi: 10.1021/jacs.0c02308 - Genetic Code Expansion Facilitates Position-Selective Labeling of RNA for Biophysical Studies
A. Hegelein*, D. Müller*, S. Größl, M. Göbel, M. Hengesbach, H. Schwalbe#
Chemistry – A European Journal, 26 (8), 1800-1810 (Highlighted with Journal Cover)
doi: 10.1002/chem.201904623 - Assembly of Proteins by Free RNA during the Early Phase of Proteostasis Stress
M. Alriquet M., A. Martínez-Limón, G. Hanspach, M. Hengesbach, G. G Tartaglia, G. Calloni, R.M. Vabulas
J Proteome Res. 2019;18(7):2835–2847.
doi:10.1021/acs.jproteome.9b00143 - Combined smFRET and NMR analysis of riboswitch structural dynamics
Bains JK*, J. Blechar*, V. de Jesus*, N. Meiser*, H. Zetzsche, B. Fürtig#, H. Schwalbe#, and M. Hengesbach#
Methods, 2019 Jan 15;153:22-34.
doi: 10.1016/j.ymeth.2018.10.004 - The Protein Microenvironment Governs the Suitability of Labeling Sites for Single-Molecule Spectroscopy of RNP Complexes.
Schmidt A, N. Altincekic, H. Gustmann, J. Wachtveitl and M. Hengesbach#
ACS Chem Biol, 2018 13(9): 2472-2483 - NMR Structural Profiling of Transcriptional Intermediates Reveals Riboswitch Regulation by Metastable RNA Conformations
Helmling, A. Wacker, M.T. Wolfinger, I.L. Hofacker, M. Hengesbach, B. Fürtig, and H. Schwalbe#
JACS, 2017 Feb. 139(7):2647-2656 - Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy
S. Warhaut, K.R. Mertinkus, P. Höllthaler, B. Fürtig,M. Heilemann, M. Hengesbach, and H. Schwalbe#
Nucleic Acids Res. 2017 Feb 15.
doi: 10.1093/nar/gkx110 - Rapid NMR screening of RNA secondary structure and binding
Helmling, S. Keyhani, F. Sochor, B. Fürtig, M. Hengesbach, and H. Schwalbe#
Journal of Biomolecular NMR 2015 Sep;63(1):67-76. - Differential scanning fluorimetry for monitoring RNA stability
Silvers, H. Keller, H. Schwalbe#, M. Hengesbach#
ChemBioChem 2015 May 4;16(7):1109-14 (Highlighted as Inside Cover) - Single molecule FRET reveals a rugged folding energy landscape for the human telomerase RNA pseudoknot domain
Hengesbach, N.-K. Kim, J. Feigon, and M.D. Stone
Angewandte Chemie International Edition, 2012 Jun 11;51(24):5876-9. - Single-molecule FRET reveals a cooperative effect of two methyl group modifications in the folding of human mitochondrial tRNALys
Yu. Kobitski, M. Hengesbach, S. Seidu-Larry, K. Dammertz, C. S. Chow, A. van Aerschot, G.U. Nienhaus and M. Helm
Chemistry & Biology, 2011 Jul 29;18(7):928-36 - Single-molecule FRET studies of counterion effects on the free energy landscape of human mitochondrial
lysine tRNA
Dammertz, M. Hengesbach, M. Helm, G.U. Nienhaus, and A. Yu.Kobitski
Biochemistry, 2011 Apr 19;50(15):3107-15 - Formation of a stalled early intermediate of Pseudouridine synthesis monitored by real-time FRET
Hengesbach, F. Voigts-Hoffmann, B. Hofmann and M. Helm
RNA, 2010 Mar;16(3):610-20.
Sculpting an RNA conformational energy landscape by a methyl group modification
- a single-molecule FRET study
Yu. Kobitski, M. Hengesbach, M. Helm, and G.U. Nienhaus
Angewandte Chemie International Edition, 2008. 47(23): p. 4326-30
(Arbeit in deutscher Sprache: Angewandte Chemie, 2008. 120(23): p. 4398-4402) - Use of DNAzymes for site-specific analysis of ribonucleotide modifications
Hengesbach*, M. Meusburger*, F. Lyko and M. Helm
RNA, 2008 Jan. 14(1): p. 180-7 - A Methyl Group Controls Conformational Equilibrium in Human Mitochondrial tRNALys
Voigts-Hoffmann, M. Hengesbach, A. Yu. Kobitski, A. van Aerschot, P. Herdewijn, G.U. Nienhaus, and M. Helm
JACS, 2007 Nov. 129(44): p. 13382-3
Reviews & Methods
- From Antisense RNA to RNA Modification: Therapeutic Potential of RNA-Based Technologies
Adachi H, Hengesbach M, Yu YT and Morais P
Biomedicines, 2021, 9
doi: 10.3390/biomedicines9050550M. - NMR Spectroscopy of Large Functional RNAs: From Sample Preparation to Low-Gamma Detection
Schnieders R, Knezic B, Zetzsche H, Sudakov A, Matzel T, Richter C, Hengesbach M, Schwalbe H and Furtig B
Curr Protoc Nucleic Acid Chem, 2020, 82, e116.
doi: 10.1002/cpnc.116 - Genetic Code Expansion Facilitates Position-Selective Modification of Nucleic Acids and Proteins
Muller D, Trucks S, Schwalbe H and Hengesbach M#
Chempluschem, 2020, 85, 1233-1243.
doi: 10.1002/cplu.202000150 - Cooperative Analysis of Structural Dynamics in RNA-Protein Complexes by Single-Molecule Forster Resonance Energy Transfer Spectroscopy
Meiser N, Fuks C and Hengesbach M
Molecules, 2020, 25,
doi: 10.3390/molecules25092057 - Strategic labelling approaches for RNA single-molecule spectroscopy
Hanspach G, Trucks S, Hengesbach M. #
RNA Biology. 2019 Mar 15:1-14.
doi: 10.1080/15476286.2019.1593093. - Single-molecule Analysis of Telomerase Structure and Function
M. Hengesbach, B.M. Akiyama, and M.D. Stone
Current Opinion in Chemical Biology, 2011 Dec;15(6):845-52. - A post-labeling Approach for the Characterization and Quantification of RNA modifications Based on Site-directed Cleavage by DNAzymes
M. Meusburger*, M. Hengesbach*, and M. Helm
Methods in Molecular Biology: DNA and RNA editing, 2011, 718; p259-70 - RNA Intramolecular Dynamics by Single-Molecule FRET
M. Hengesbach, A. Kobitski, F. Voigts-Hoffmann, C. Frauer, G.U. Nienhaus, and M. Helm
Current Protocols in Nucleic Acid Chemistry, Sep 2008, Chapter 11; Unit 11.12 - Exploring the folding free energy landscape of small RNA molecules by single-pair Förster resonance energy transfer
A. Yu. Kobitski, A. Nierth, M. Hengesbach, A. Jäschke, M. Helm, and G.Ulrich Nienhaus
Biophysical Reviews and Letters, 2008. 3(4): p. 439-457.
NMR assignment publications:
- 1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.
Biomol NMR Assign. 2022 Apr;16(1):17-25.
doi: 10.1007/s12104-021-10053-4 - 1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2
Richter C, Hohmann KF, Toews S, Mathieu D, Altincekic N, Bains JK, Binas O, Ceylan B, Duchardt-Ferner E, Ferner J, Fürtig B, Grün JT, Hengesbach M, Hymon D, Jonker HRA, Knezic B, Korn SM, Landgraf T, Löhr F, Peter SA, Pyper DJ, Qureshi NS, Schlundt A, Schnieders R, Stirnal E, Sudakov A, Vögele J, Weigand JE, Wirmer-Bartoschek J, Witt K, Wöhnert J, Schwalbe H, Wacker A.
Biomol NMR Assign. 2021 Oct;15(2):467-474.
doi: 10.1007/s12104-021-10047-2. - 1H, 13C and 15N and (31)P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2
Vogele J, Ferner JP, Altincekic N, Bains JK, Ceylan B, Furtig B, Grun JT, Hengesbach M, Hohmann KF, Hymon D, Knezic B, Lohr F, Peter SA, Pyper D, Qureshi NS, Richter C, Schlundt A, Schwalbe H, Stirnal E, Sudakov A, Wacker A, Weigand JE, Wirmer-Bartoschek J, Wohnert J and Duchardt-Ferner E
Biomol NMR Assign, 2021,
doi: 10.1007/s12104-021-10026-7 - 1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2
Schnieders R, Peter SA, Banijamali E, Riad M, Altincekic N, Bains JK, Ceylan B, Furtig B, Grun JT, Hengesbach M, Hohmann KF, Hymon D, Knezic B, Oxenfarth A, Petzold K, Qureshi NS, Richter C, Schlagnitweit J, Schlundt A, Schwalbe H, Stirnal E, Sudakov A, Vogele J, Wacker A, Weigand JE, Wirmer-Bartoschek J and Wohnert J
Biomol NMR Assign, 2021, 15, 203-211.
doi: 10.1007/s12104-021-10007-w - 1H, 13C and 15N backbone chemical shift assignments of SARS-CoV-2 nsp3a
Salvi N, Bessa LM, Guseva S, Camacho-Zarco A, Maurin D, Perez LM, Malki A, Hengesbach M, Korn SM, Schlundt A, Schwalbe H and Blackledge M
Biomol NMR Assign, 2021, 15, 173-176.
doi: 10.1007/s12104-020-10001-8 - 1H, 13C and 15N backbone chemical shift assignments of the C-terminal dimerization domain of SARS-CoV-2 nucleocapsid protein
Korn SM, Lambertz R, Furtig B, Hengesbach M, Lohr F, Richter C, Schwalbe H, Weigand JE, Wohnert J and Schlundt A
Biomol NMR Assign, 2021, 15, 129-135.
doi: 10.1007/s12104-020-09995-y - 1H, 13C and 15N backbone chemical shift assignments of coronavirus-2 non-structural protein Nsp10
Kubatova N, Qureshi NS, Altincekic N, Abele R, Bains JK, Ceylan B, Ferner J, Fuks C, Hargittay B, Hutchison MT, de Jesus V, Kutz F, Wirtz Martin MA, Meiser N, Linhard V, Pyper DJ, Trucks S, Furtig B, Hengesbach M#, Lohr F, Richter C, Saxena K, Schlundt A, Schwalbe H#, Sreeramulu S, Wacker A, Weigand JE, Wirmer-Bartoschek J and Wohnert J
Biomol NMR Assign, 2021, 15, 65-71.
doi: 10.1007/s12104-020-09984-1 - 1H, 13C and 15N backbone chemical shift assignments of the apo and the ADP-ribose bound forms of the macrodomain of SARS-CoV-2 non-structural protein 3b
Cantini F, Banci L, Altincekic N, Bains JK, Dhamotharan K, Fuks C, Furtig B, Gande SL, Hargittay B, Hengesbach M, Hutchison MT, Korn SM, Kubatova N, Kutz F, Linhard V, Lohr F, Meiser N, Pyper DJ, Qureshi NS, Richter C, Saxena K, Schlundt A, Schwalbe H, Sreeramulu S, Tants JN, Wacker A, Weigand JE, Wohnert J, Tsika AC, Fourkiotis NK and Spyroulias GA
Biomol NMR Assign, 2020, 14, 339-346.
doi: 10.1007/s12104-020-09973-4 - 1H, 13C and 15N backbone chemical shift assignments of the nucleic acid-binding domain of SARS-CoV-2 non-structural protein 3e
Korn SM, Dhamotharan K, Furtig B, Hengesbach M, Lohr F, Qureshi NS, Richter C, Saxena K, Schwalbe H, Tants JN, Weigand JE, Wohnert J and Schlundt A
Biomol NMR Assign, 2020, 14, 329-333.
doi: 10.1007/s12104-020-09971-6
Book Chapters
- Handbook of RNA Biochemistry, 2nd edition, 2014
Editors: Roland K. Hartmann, Albrecht Bindereif, Astrid Schön, Eric Westhof
Chapter „RNA Ligation“, J.J. Turunen, L.V. Pavlova, M. Hengesbach, M. Helm, S. Müller, R.K. Hartmann, and M.J. Frilander
Commentaries
- The how & why of looking at individual RNAs
Hengesbach M. #
RNA Biol. 2019;16(9):1075–1076. - Structural basis for regulation of ribosomal RNA 2'-O-methylation
M. Hengesbach, H. Schwalbe#
Angewandte Chemie International Edition, 2014 Feb 10;53(7):1742-4
(Article in German: Angewandte Chemie, 2014. 126(7): p. 1770-1772)
Editorial activities & Outreach
- The COVID19-NMR Consortium: A Public Report on the Impact of this New Global Collaboration.
Angew Chem Int Ed Engl. 2023 Mar 27;62(14):e202217171.
doi: 10.1002/anie.202217171 - Einzelmolekül-FRET-Spektroskopie
Trendberichte, Nachrichten aus der Chemie August 2021 - Gemeinschaftlich in Krisenzeiten: NMR-Strukturbiologie gegen COVID-19.
Schlundt A, Wirtz MA, Knezic B, Hengesbach M, Fürtig B, Weigand JE, Wöhnert J, Ferner J, Saxena K, Wacker A, Richter C, Sreeramulu S, Wirmer-Bartoschek J, Schwalbe H; Im Namen von Mehr als 50 Wissenschaftlerinnen und Wissenschaftlern in Frankfurt und Darmstadt.
Biospektrum (Heidelb). 2020;26(4):440-441.
doi: 10.1007/s12268-020-1396-0 - Single molecule techniques – Special focus issue in RNA Biology 04/2019
Editor: M. Hengesbach
*: Joint authorship
#: Corresponding authorship